Will I lose the "Kingsoul" charm?
The Hollow Knight Wiki states:
(Spoilers for the 2nd and 3rd endings!)
This charm is primarily only equipped to unlock the secret area at the bottom of the Abyss, after which it is permanently replaced by Void Heart.
Similarly, on the wiki page about the other charm, it states:
This charm replaces Kingsoul and cannot be unequipped. However, it no longer has Kingsoul's effects, nor does it use any notches.
Does this mean that once I unlock the Void Heart charm, I can no longer use the Kingsoul charm?
From my own playthrough, I can tell you that you permanently lose the Kingsoul charm as soon as you unlock the Void Heart charm... Void Heart even replaces Kingsoul in the Charm screen.
Having said that, I haven't played through the game since it originally launched and I'm not sure if the Hidden Dreams, Grimm Troupe, or the (currently in beta) Lifeblood DLCs have changed this. I suspect they haven't since the event where you gain the Void Heart is a major plot event.