How can I repeatedly take a screenshot of a specific area in OS X?

Under OS X, I need to take a screenshot of the same specific screen area. I know I can press hotkeys to grab the entire screen, or an area of the screen, but then I always have to select it.

Can I configure OS X to always create a screenshot of the same part of the screen?

You can use the screencapture command line utility. It includes (amongst others) the following options:

-x         do not play sounds
-R<x,y,w,h> capture screen rect
files   where to save the screen capture, 1 file per screen

So, in your case, to grab a screenshot with the top left corner at 20/20, creating a 640x380 window:

screencapture -x -R20,20,640,380 ~/Desktop/test.png

You could put this command into your crontab to have it execute repeatedly, or simply use a loop that runs in a Terminal window. In that case, we will create a screenshot every second (sleep 1), and the output file will be named screen_YYMMDDTHHMMSS accordingly.

while [ 1 ]; do 
  date=$(date "+%Y%m%dT%H%M%S")
  screencapture -x -R20,20,640,380 ~/Desktop/"screen_${date}.png"
  sleep 1
done

You could also create an Automator action or AppleScript to run a shell script like the above. For example, open Script Editor, then paste this:

do shell script "date=$(date '+%Y%m%dT%H%M%S'); screencapture -x -R20,20,640,380 ~/Desktop/screen_${date}.png"

It should look like this:

Save that script and use FastScripts to easily assign a keyboard shortcut.

If you are open to using an app/utility, I would highly recommend Skitch:

https://evernote.com/skitch/

It has a shortcut for "Previous Snapshot Area."

(In addition you can capture a selection using a timer, capture the whole screen, just a window, or just a menu.)

It's free and has a ton of features including editing / annotations, quick and easy sharing, etc.