Does it have an impact on the game whether I check "Investigate" options or not?

I played through Mass Effect in the past and I know what dialogues a lot of the "Investigate" options hold in conversations.

I was wondering whether they have an impact on the future dialogue options or on the story or not? Does it change the game in any way if I click them all or it is just flavour text like in Still Life?

In the first two games of Mass Effect I did almost all the investigation options and never saw a paragon/renegade choice. I've only played the first one through once and the second one through twice, but I reached full renegade without any investigation on my second playthrough of ME2.

I think there were some paragon/renegade investigation options in the third game.