fileReader.readAsBinaryString to upload files

(Following is a late but complete answer)

FileReader methods support

FileReader.readAsBinaryString() is deprecated. Don't use it! It's no longer in the W3C File API working draft:

void abort();
void readAsArrayBuffer(Blob blob);
void readAsText(Blob blob, optional DOMString encoding);
void readAsDataURL(Blob blob);

NB: Note that File is a kind of extended Blob structure.

Mozilla still implements readAsBinaryString() and describes it in MDN FileApi documentation:

void abort();
void readAsArrayBuffer(in Blob blob); Requires Gecko 7.0
void readAsBinaryString(in Blob blob);
void readAsDataURL(in Blob file);
void readAsText(in Blob blob, [optional] in DOMString encoding);

The reason behind readAsBinaryString() deprecation is in my opinion the following: the standard for JavaScript strings are DOMString which only accept UTF-8 characters, NOT random binary data. So don't use readAsBinaryString(), that's not safe and ECMAScript-compliant at all.

We know that JavaScript strings are not supposed to store binary data but Mozilla in some sort can. That's dangerous in my opinion. Blob and typed arrays (ArrayBuffer and the not-yet-implemented but not necessary StringView) were invented for one purpose: allow the use of pure binary data, without UTF-8 strings restrictions.

XMLHttpRequest upload support

XMLHttpRequest.send() has the following invocations options:

void send();
void send(ArrayBuffer data);
void send(Blob data);
void send(Document data);
void send(DOMString? data);
void send(FormData data);

XMLHttpRequest.sendAsBinary() has the following invocations options:

void sendAsBinary(   in DOMString body );

sendAsBinary() is NOT a standard and may not be supported in Chrome.

Solutions

So you have several options:

1. send() the FileReader.result of FileReader.readAsArrayBuffer ( fileObject ). It is more complicated to manipulate (you'll have to make a separate send() for it) but it's the RECOMMENDED APPROACH.
2. send() the FileReader.result of FileReader.readAsDataURL( fileObject ). It generates useless overhead and compression latency, requires a decompression step on the server-side BUT it's easy to manipulate as a string in Javascript.
3. Being non-standard and sendAsBinary() the FileReader.result of FileReader.readAsBinaryString( fileObject )

MDN states that:

The best way to send binary content (like in files upload) is using ArrayBuffers or Blobs in conjuncton with the send() method. However, if you want to send a stringifiable raw data, use the sendAsBinary() method instead, or the StringView (Non native) typed arrays superclass.

Use fileReader.readAsDataURL( fileObject ), this will encode it to base64, which you can safely upload to your server.

The best way in browsers that support it, is to send the file as a Blob, or using FormData if you want a multipart form. You do not need a FileReader for that. This is both simpler and more efficient than trying to read the data.

If you specifically want to send it as multipart/form-data, you can use a FormData object:

var xmlHttpRequest = new XMLHttpRequest();
xmlHttpRequest.open("POST", '/pushfile', true);
var formData = new FormData();
// This should automatically set the file name and type.
formData.append("file", file);
// Sending FormData automatically sets the Content-Type header to multipart/form-data
xmlHttpRequest.send(formData);

You can also send the data directly, instead of using multipart/form-data. See the documentation. Of course, this will need a server-side change as well.

// file is an instance of File, e.g. from a file input.
var xmlHttpRequest = new XMLHttpRequest();
xmlHttpRequest.open("POST", '/pushfile', true);

xmlHttpRequest.setRequestHeader("Content-Type", file.type);

// Send the binary data.
// Since a File is a Blob, we can send it directly.
xmlHttpRequest.send(file);

For browser support, see: http://caniuse.com/#feat=xhr2 (most browsers, including IE 10+).