Uniquate = Eliminate or replace duplicates, make unique?

Solution 1:

Your idea 'uniquate' is possible but few would understand its intention without a lot of context.

uniquify

is not an officially recognized term, but uses the very productive and understandable suffix '-ify' meaning 'to make'. However this particular construction sounds too informal as a neologisms to be appropriate.

A single term is not necessary. You can say what is happening:

remove duplicates

Solution 2:

How about disambiguate? Webster’s dictionary defines it to be:

to establish a single semantic or grammatical interpretation for.