Solution 1:

1) $c_1(X):=c_1(TX)=c_1(\wedge^n(TX))=-c_1(K_X)$ where $TX$ is the holomorphic tangent bundle as in Georges's comment.

2) If $K_X$ is ample, then $c_1(K_X)>0$ in the sense that $c_1(K_X).C>0$ for all irreducible holomorphic curve $C$ in $X$. This is because some positive multiple of $K_X$ is very ample, hence positive.