Block a registry key to prevent rewriting or deleting accidentally or intentionally using the command line

I need to block this key to prevent deletion or modification by malware.

HKLM\SOFTWARE\Microsoft\Windows NT\CurrentVersion\Image File Execution Options\wscript.exe

What command line can help me to do this?

As per the answers in the link in your question, the easiest way to do this is to remove the Edit permissions from SYSTEM and the Administrators groups and the key should then effectively be read only.

You can do this from the command line with regini by following the procedure below.

Warning:

• I would be very tempted to back up your whole system before making such changes (you may feel comfortable just backing up the registry - see below).

• The instructions below contain steps that tell you how to modify the registry. However, serious problems might occur if you modify the registry incorrectly.

• For added protection, back up the registry before you modify it. Then, you can restore the registry if a problem occurs.

• For more information see How to back up and restore the registry in Windows

1. Create a file (for example block.txt) with the following contents:

    HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\Windows NT\CurrentVersion\Image File Execution Options\wscript.exe [2 19]
   

2. Execute the block.txt script using the following command, run from an elevated (Administrator) cmd shell:

    regini block.txt
   

Notes:

• The regini command must be run from an elevated (Administrator) cmd shell, otherwise it will fail (an no error will be displayed).

• The permission entry is set to [2 19] which is Administrators Read Access and SYSTEM Read Access.

• You need to think carefully whether other user groups also require read access.

  Using a permission entry of [2 8 19] will in addition also allow standard users (and administrator accounts with filtered user token) read access for the key.

• You may need to change the permissions of the parent key to prevent sub-keys being deleted (I haven't verified this).

Further Reading

• An A-Z Index of the Windows CMD command line - An excellent reference for all things Windows cmd line related.
• regini - Change Registry Permissions.
• How to: Use a Script to Change Registry Permissions from the Command Line