The Space $C(\Omega,\mathbb{R})$ has a Predual?
Solution 1:
Firstly, let me just point out that every compact metric space is automatically separable.
Secondly, note that $c_0$ does not embed into any separable dual space, hence neither does any Banach space containing a subspace isomorphic to $c_0$. Since every $C(K)$ space ($K$ compact Hausdorff) contains a subspace isomorphic to $c_0$, no $C(K)$ space embeds isomorphically into a separable dual space. In particular, since metrizability of $K$ is equivalent to $C(K)$ being norm separable, the answer to your question is always no.
For a reference for all of the above claims, look up $C(K)$ and $c_0$ in the index of Albiac and Kalton's book Topics in Banach Space Theory.