Issue with Game of Life

I suspect¹ the problem with your code is that it is setting the cells to living or dead as soon as the neighbors are checked. That caused my early variants of this code to fail. That change of state has to be delayed until the entire grid (biosphere) has been checked.

This example shows typical Game of Life behavior.

1. Note that "suspicions ain't answers", so it is best to post an SSCCE.

import java.awt.*;
import java.awt.event.*;
import javax.swing.*;
import java.util.Random;

public class GameOfLife extends JPanel {

    private final int row, col;
    private boolean isLiving;

    public static Random random = new Random();

    public GameOfLife(int r, int c) {
        this.row = r;
        this.col = c;
        MouseListener listener = new MouseAdapter() {
            @Override
            public void mouseClicked(MouseEvent e) {
                isLiving = !isLiving;
                repaint();
            }
        };
        this.addMouseListener(listener);
        isLiving = random.nextBoolean();
    }

    public boolean isAlive(int neighbors) {
        boolean alive = false;
        if (this.isLiving) {
            if (neighbors < 2) {
                alive = false;
            } else if (neighbors == 2 || neighbors == 3) {
                alive = true;
            } else if (neighbors > 3) {
                alive = false;
            }
        } else {
            if (neighbors == 3) {
                alive = true;
            }
        }
        return alive;
    }

    public void setAlive(boolean alive) {
        isLiving = alive;
    }

    public boolean isLiving() {
        return this.isLiving;
    }

    @Override
    public void paintComponent(Graphics g) {
        super.paintComponent(g);
        if (this.isLiving) {
            g.fillRect(0, 0, getWidth() - 1, getHeight() - 1);
        } else {
            g.drawRect(0, 0, getWidth() - 1, getHeight() - 1);
        }
    }

    public static void main(String[] args) {
        final int s = 40;
        final GameOfLife[][] biosphere = new GameOfLife[s][s];
        final JPanel gui = new JPanel(new GridLayout(s, s, 2, 2));
        for (int ii = 0; ii < s; ii++) {
            for (int jj = 0; jj < s; jj++) {
                GameOfLife cell = new GameOfLife(ii, jj);
                cell.setPreferredSize(new Dimension(10, 10));
                gui.add(cell);
                biosphere[ii][jj] = cell;
            }
        }

        ActionListener al = (ActionEvent ae) -> {
            boolean[][] living = new boolean[s][s];
            for (int ii = 0; ii < s; ii++) {
                for (int jj = 0; jj < s; jj++) {
                    int top = (jj > 0 ? jj - 1 : s - 1);
                    int btm = (jj < s - 1 ? jj + 1 : 0);
                    int lft = (ii > 0 ? ii - 1 : s - 1);
                    int rgt = (ii < s - 1 ? ii + 1 : 0);
                    int neighbors = 0;
                    if (biosphere[ii][top].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[ii][btm].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[lft][top].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[lft][btm].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[lft][jj].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[rgt][jj].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[rgt][top].isLiving()) {
                        neighbors++;
                    }
                    if (biosphere[rgt][btm].isLiving()) {
                        neighbors++;
                    }
                    living[ii][jj] = biosphere[ii][jj].isAlive(neighbors);
                }
            }
            for (int ii = 0; ii < s; ii++) {
                for (int jj = 0; jj < s; jj++) {
                    biosphere[ii][jj].setAlive(living[ii][jj]);
                }
            }
            gui.repaint();
        };

        Timer timer = new Timer(50, al);
        timer.start();

        JOptionPane.showMessageDialog(null, gui);
        timer.stop();
    }
}