Moving Active Directory computer to another OU - What are the affects?

We have all our computers currently in the root OU for computers. I'd like to start cleaning this up so I can track changes going forward. (Currently too many people have the admin password but that's another issue)

If I create a sub OU for say servers, and then move all the server into it, another for users that I know exist, another for very old system machines etc will there be anything noticeable from the user side of things?

We currently have no working GPO's assigned to the groups and the remaining ones are being checked and removed so I don't think that'll cause any issues.

If you have no GPO setup at all, then nothing should change. This will just be a sorting within the AD for you. Once this is done it's a great way to then start to use GPO's to lock down things.

When we did something similar we had our LDAP users start getting failures as the DNs had moved on them. It was an in house ap though, so you may not have that problem.