Checklists for instruction - direct or second person?

For instruction within a professional org, we utilize checklists for the specific parties to self-assess their "products". Because there is some unfamiliarity with the subject matter, we initially approached the checklists using second person to feel more approachable.

Example: During keyboard testing, can you clearly see where the focus is on the page at all times?

However, I think it may be better to be more direct, removing second person as checklists are instructional and direct.

Example: During keyboard testing, is focus visible at all times?

Is there a rule of thumb that applies here?

Which is preferred really depends on the culture at your organization. However, saying it concisely using the imperative mood is both more efficient to write and has a stronger impact (e.g. your second example). It's a checklist -- people do not expect niceties, but quick help to get something done.