calc() not working within media queries

@media screen and (max-width: calc(2000px-1px)) { 
  .col { width: 200px; }
}

The value after subtraction should be 1999px, however it does not seem to be working. If I manually change it to 1999px it works fine, so I know it's not a problem with my CSS. Is calc not supported within media queries, or am I doing something wrong?

ANSWER WAS EDITED 13.02.2018:

Using calc in media queries is supported by the spec, but support was only implemented by browsers recently (February 2018). Currently, calc in media queries is supported by Safari Technology Preview 49+, Chrome 66+, and Firefox 59+. See MDN's calc() page for the most up-to-date information.

Support for use of calc() in media queries is browser-dependent HOWEVER the use of mixed units (e.g. em and px at same time) have limited or no current support. Please take a look at this JSFiddle (which tests 4 current browsers - Chrome 80.0.3987.163, Opera 67.0.3575.115, Firefox 74.0.1, and Microsoft Edge 44.18362.449.0) to verify.

For example, these media queries are valid for certain browsers (Chrome 80.0.3987.163, Opera 67.0.3575.115, and Firefox 74.0.1 but NOT Microsoft Edge 44.18362.449.0)

@media (min-width:calc(2em - 1em)) { div { color: green } } // valid

@media (min-width:calc(2px - 1px)) { div { color: green } } // valid

whereas this media query is only valid in Firefox 74.0.1

@media (min-width:calc(1em - 1px)) { div { color: green } } // different units are "mixed" in same calc() -----> invalid except Firefox

UPDATE December 2020: Other functions within the calc() family of functions (that is, functions which perform an operation on one or more calc-sums) can also be used within a media query. This JSFiddle demonstrates the utility of the comparison functions max(), min(), and clamp(). These functions are currently supported within a media query by the browsers:

1. Chrome 87
2. Edge 87
3. Firefox 82
4. Opera 72

However, using mixed units within each comparison function is only supported by the following browsers (from the list above):

1. Firefox 82

Pinal's answer is great, but your CSS wouldn't work anyways. You need spaces separating your units. In CSS 2000px-1px would be considered a single value, which obviously isn't a valid unit. It should be 2000px - 1px.

I'm currently using Chrome 66 and calc works fine in media queries.