Solution 1:

What about checking the "Read-only" box in the main folder's Properties and then applying to all subfolders?

Solution 2:

My best option is use DISKPART form windows

DISKPART

LIST DISK (To check which flash drive listed as disk in windows)

SELECT DISK 1 (It should be disk 1 if you have single hardisk attached to computer, disks naming starts from 0 but partition starts from 1)

ATTRIBUTES DISK SET READONLY [And its done ;-) ]