parsing a tab-separated file in Python

You can use the csv module to parse tab seperated value files easily.

import csv

with open("tab-separated-values") as tsv:
    for line in csv.reader(tsv, dialect="excel-tab"): #You can also use delimiter="\t" rather than giving a dialect.
        ... 

Where line is a list of the values on the current row for each iteration.

Edit: As suggested below, if you want to read by column, and not by row, then the best thing to do is use the zip() builtin:

with open("tab-separated-values") as tsv:
    for column in zip(*[line for line in csv.reader(tsv, dialect="excel-tab")]):
        ...

I don't think any of the current answers really do what you said you want. (Correction: I now see that @Gareth Latty / @Lattyware has incorporated my answer into his own as an "Edit" near the end.)

Anyway, here's my take:

Say these are the tab-separated values in your input file:

1   2   3   4   5
6   7   8   9   10
11  12  13  14  15
16  17  18  19  20

then this:

with open("tab-separated-values.txt") as inp:
    print( list(zip(*(line.strip().split('\t') for line in inp))) )

would produce the following:

[('1', '6', '11', '16'), 
 ('2', '7', '12', '17'), 
 ('3', '8', '13', '18'), 
 ('4', '9', '14', '19'), 
 ('5', '10', '15', '20')]

As you can see, it put the k-th element of each row into the k-th array.

Like this:

>>> s='1\t2\t3\t4\t5'
>>> [x for x in s.split('\t')]
['1', '2', '3', '4', '5']

For a file:

# create test file:
>>> with open('tabs.txt','w') as o:
...    s='\n'.join(['\t'.join(map(str,range(i,i+10))) for i in [0,10,20,30]])
...    print >>o, s

#read that file:
>>> with open('tabs.txt','r') as f:
...    LoL=[x.strip().split('\t') for x in f]
... 
>>> LoL
[['0', '1', '2', '3', '4', '5', '6', '7', '8', '9'], 
 ['10', '11', '12', '13', '14', '15', '16', '17', '18', '19'], 
 ['20', '21', '22', '23', '24', '25', '26', '27', '28', '29'], 
 ['30', '31', '32', '33', '34', '35', '36', '37', '38', '39']]
>>> LoL[2][3]
23

If you want the input transposed:

>>> with open('tabs.txt','r') as f:
...    LoT=zip(*(line.strip().split('\t') for line in f))
... 
>>> LoT[2][3]
'32'

Or (better still) use the csv module in the default distribution...

You can easily do it like this way by python pandas pd.read_csv ('file_name.tsv', sep='\t')

[Note: need to install pandas with this command pip install pandas]